In the present report, therapeutic regDC EXO were analyzed by high throughput proteomics, with non-therapeutic EXO from immature DCs and mature DCs as controls, to identify shared and distinct proteins and potential off-target proteins, as corroborated by immunoblot. However, the on- and off-target effects of these therapeutic regDC EXO and how target signaling in acceptor cells is activated is unclear. We showed a key role for encapsulated TGFβ1 in promoting a bone sparing immune response. Previously we documented the efficacy of immunoregulatory DCs EXO (regDCs EXO) as immunotherapy for inflammatory bone disease, in an in-vivo model. DC EXO are eobiotic, meaning they are well-tolerated by the host moreover, they can be custom-tailored for immune-regulatory or -stimulatory functions, thus presenting attractive opportunities for immune therapy.

8Division of Infectious Diseases, Department of Medicine, Medical College of Georgia at Augusta University, Augusta, GA, United Statesĭendritic cell (DC)-derived exosomes (DC EXO), natural nanoparticles of endosomal origin, are under intense scrutiny in clinical trials for various inflammatory diseases.

7Division of Pulmonary and Critical Care Medicine, Medical College of Georgia at Augusta University, Augusta, GA, United States.

6Vascular Biology Center, Medical College of Georgia at Augusta University, Augusta, GA, United States.

5Center of Biotechnology and Genomic Medicine, at Augusta University, Augusta, GA, United States.

4Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, GA, United States.

3Georgia Cancer Center, Department of Biochemistry and Molecular Biology, at Augusta University, Augusta, GA, United States.

2Department of Endodontics, College of Dentistry, Ainshams University, Cairo, Egypt.

1Department of Periodontics, Dental College of Georgia at Augusta University, Augusta, GA, United States.

Elawady 1, Mark Hamrick 4, Yutao Liu 4, Wenbo Zhi 5, Rudolf Lucas 6,7, Jose Vazquez 8 and Christopher W. Mahmoud Elashiry 1†, Ranya Elsayed 1†, Mohamed M.

Origin 8 cutler 7